5-HT(2A) receptors in the prelimbic cortex VIP-expressing interneurons: A mechanism for psychedelic-induced innate fear attenuation.
Fear-related psychiatric disorders such as phobias and anxiety are insufficiently treated by current therapies. Psychedelics targeting 5-HT₂A receptors show promise in modulating fear circuits, but their specific neurobiological substrates remain unclear. This study investigates
- Authors
- Wang Y, Yao Y, Su R, Yan H
- Journal
- British journal of pharmacology
- Published
- 2026-07-01
- DOI
- 10.1111/bph.70307
- PMID
- 41761564
- Content type
- Paper
- Authority
- Academic
Abstract
Fear-related psychiatric disorders such as phobias and anxiety are insufficiently treated by current therapies. Psychedelics targeting 5-HT₂A receptors show promise in modulating fear circuits, but their specific neurobiological substrates remain unclear. This study investigates the effects of 4-acetoxy-N,N-dimethyltryptamine (4-AcO-DMT), a psychedelic compound, on innate fear responses and elucidates its underlying cortical microcircuits, focusing on predator odour-evoked 22-kHz ultrasonic vocalizations (USVs) in rats. Using an optimized paradigm of 2,4,5-trimethylthiazoline (TMT)-induced 22-kHz USVs in rats, the effects of 4-AcO-DMT were evaluated, and the dose-response relationships were investigated. In behavioural experiments, we demonstrated that 4-AcO-DMT suppresses 22-kHz USVs by reducing fear-related response in rats. Brain-wide EGR1 expression and targeted brain region lesions were employed to identify the brain region through which 4-AcO-DMT acts. Specific neuronal ablation and chemogenetic manipulation were applied to determine the types of neurons modulated by 4-AcO-DMT, and receptor antagonism and localized receptor knockout strategies were used to identify the receptor subtypes involved. 4-AcO-DMT significantly suppressed TMT evoked 22-kHz USVs via 5-HT₂A receptor activation. This fear attenuation was mediated by recruitment of VIP interneurons in the prelimbic cortex (PrL), as chemogenetic activation of these cells abolished the behavioural effect. A cortical microcircuit mechanism-5-HT₂A receptor-driven vasoactive intestinal polypeptide (VIP) interneuron engagement-is critical to 4-AcO-DMT -induced suppression of innate fear responses. Our study reveals that 5-HT₂A receptor activation by 4-AcO-DMT suppresses innate fear through VIP interneuron-mediated modulation in the prelimbic cortex, advancing the neurobiological understanding of serotonergic psychedelics. This article is part of a themed issue Emerging Therapeutic Opportunities for Psychedelic and Related Drugs. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v183.14/issuetoc.
Citation
Wang Y, Yao Y, Su R, Yan H (2026) 5-HT(2A) receptors in the prelimbic cortex VIP-expressing interneurons: A mechanism for psychedelic-induced innate fear attenuation.. British journal of pharmacology. doi:10.1111/bph.70307
DOI: 10.1111/bph.70307
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